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Media Supplements, Freezing Media & other Cell Culture Reagents
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Cell Dissociation Reagents
Trypsin 0.5% - EDTA 5.3 mM (10X), liquid
Trypsin is an enzyme preparation derived from porcine pancreas, generally used for the isolation and dissociation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary for the successful isolation of cells depends primarily on the cell type and the age of the culture.
Trypsin 0.5% is a 10X concentrate of Trypsin 0.05%, the trypsin solution of choice for more sensitive cell lines. EDTA (Ethylenediaminetetraacetic acid), a chelating agent that neutralizes calcium and magnesium ions, is added to increase the enzymatic activity on the cells and to facilitate release of individual cells from the culture vessel. Calcium and magnesium ions enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. This trypsin solution contains 5.0 gm/L of trypsin (1:250) and 2.0 gm/L EDTA•4Na in normal saline solution and must be diluted in an appropriate buffer before use.
Technical Information
• MSDS - Trypsin 0.5%-EDTA 5.3mM (10X), liquid
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